What is PBST and what is it used for?

PBST, or Phosphate Buffered Saline with Tween-20, is a solution commonly used in biochemistry and molecular biology. It maintains a stable pH while providing an essential ionic environment for cells. This buffer also contains Tween-20, a non-ionic detergent, that is effective for blocking non-specific binding during various assays. Researchers often use PBST for washing in ELISA procedures, immunostaining, and other experiments requiring a stable buffered environment.

What is the impact of Tween-20 in PBST solutions?

Tween-20 enhances the functionality of PBST by reducing non-specific binding sites, which is crucial during laboratory experiments where precision is vital. This detergent helps to maintain the structural integrity of proteins and other biological molecules while ensuring that only specific interactions occur during assays. For example, in ELISA tests, Tween-20 improves signal detection by decreasing background noise.

How should PBST be prepared and stored?

PBST should ideally be prepared by diluting the 10X concentrated stock with distilled water to achieve the desired concentration. It should be stored at 4°C for short-term use or frozen for long-term storage. It is important to ensure the buffer is mixed thoroughly and, when using, to bring it to room temperature before use to avoid temperature-induced variations that can affect experimental results.

Can PBST be used in Western blotting?

Yes, PBST is commonly used in Western blotting as a wash buffer and for diluting antibodies. Its composition helps to minimize background noise and enhances the clarity of the bands in the final visual results. For improved specificity in Western blots, PBST is often utilized after protein transfer processes to wash away unbound antibodies, ensuring that only those that specifically bind to the target protein remain.

What are the advantages of using a phosphate buffer in PBST?

Using a phosphate buffer in PBST provides several advantages, primarily in maintaining a stable pH during various biological reactions. Phosphate buffers are compatible with many biological systems and minimize ionic strength fluctuations. This stability is critical for experiments involving proteins or nucleic acids that are sensitive to changes in pH, ensuring reliable and reproducible results in applications such as cell culture and various assay techniques.

Is PBST suitable for cell culture applications?

PBST can be suitable for certain cell culture applications, especially those involving the washing or treatment of cells prior to further analysis. However, it’s essential to consider that while PBST maintains pH and reduces non-specific binding, it also contains Tween-20, which could affect cell viability. In cases where cell health is paramount, researchers often switch to a more benign buffer without detergents for handling live cell assays.

Can PBST be used in immunofluorescence staining?

Absolutely, PBST is frequently utilized in immunofluorescence staining protocols. The buffer allows for effective permeabilization and reduces background fluorescence caused by non-specific antibody binding. Successfully utilizing PBST in the washing stages of immunofluorescence ensures that only the specific binding of antibodies to the target antigens is visualized, allowing clearer and more precise imaging results.

What precautions should be taken when using PBST?

When using PBST, it’s important to check the expiration date as the buffer solutions can degrade over time. Additionally, to prevent contamination, always utilize sterile techniques when preparing and handling the buffer. If working with sensitive proteins or antibodies, ensure that the buffer components do not adversely affect stability or activity, as some proteins can be sensitive even to low concentrations of detergents.

Where can I buy PBST [10X]?

You can purchase PBST [10X] from Ubuy in Paraguay. They offer a wide range of laboratory reagents and supplies suitable for various research needs. Ubuy ensures that you receive high-quality products that meet industry standards, making it easier for you to obtain the essential materials for your experiments and research projects.

What is the shelf life of PBST?

The shelf life of PBST can vary depending on the storage conditions and the presence of components like Tween-20. Generally, when stored correctly at 4°C, it can remain stable for several months. However, it's good practice to check for any visible signs of contamination or pH changes before use. Always maintain the buffer in clean, sterile containers to prolong its usability for your laboratory experiments.

¿Cómo comprar PBST [10X]; Phosphate Buffered Saline with Tween-20 en línea desde Ubuy?

Es fácil comprar PBST [10X]; Phosphate Buffered Saline with Tween-20 en línea desde Ubuy.. Solo tiene que buscar el producto, elegir su método de envío al pagar y recibirlo en su ubicación.

¿Está PBST [10X]; Phosphate Buffered Saline with Tween-20 disponible para comprar en línea en Paraguay?

Sí, en Ubuy Paraguay, este producto está disponible para que lo compre a un precio razonable.. El PBST [10X]; Phosphate Buffered Saline with Tween-20 no está disponible localmente, pero puede confiar en nosotros con nuestros servicios de envío exprés.

¿Cuánto tiempo se tarda en obtener el producto después de realizar el pedido?

El tiempo de entrega de su producto pedido varía según lo que haya pedido y el método de envío que haya elegido.. El tiempo de entrega estimado se menciona durante el proceso de pago, así que no se preocupe mientras compra.

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PBST [10X]; Phosphate Buffered Saline with Tween-20 Solution; 80mM Na2HPO4, 1.5M NaCl, 20mM KH2PO4, 30mM KCl, 0.5% Tween-20, pH 7.4-1 L

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PBST [10X]; Phosphate Buffered Saline with Tween-20 Solution; 80mM Na2HPO4, 1.5M NaCl, 20mM KH2PO4, 30mM KCl, 0.5% Tween-20, pH 7.4-1 L

Nº de artículo: 127610908

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What Stands Out

Versatile Buffer Solution

PBST 10X is ideal for a variety of lab applications, ensuring optimal pH levels to maintain the integrity of biological samples.

Detergent Inclusion

Contains 0.5% Tween-20, enhancing solubility and reducing non-specific binding in immunoassays, improving the accuracy of experimental results.

High Concentration

10X formulation allows for easy dilution, providing flexibility for different experimental needs while ensuring cost-effectiveness with a single concentrated solution.

Detalles de producto

Container Type	Bottle
Item Form	Liquid
Item Weight	16 ounces (453.6 grams)
Brand Name	eCon Lab Supply
Manufacturer	Ciro Mfg
Included Components	Bottle
Part Number	23.105.11
Brand	eCon Lab Supply
Package Information	Bottle

Who Should Buy?

Laboratory Researchers
Ideal for biologists conducting experiments requiring a buffered saline solution with surfactant properties for effective washing.
Immunoassay Users
Excellent for those performing ELISA or Western blotting, where non-specific binding reduction is crucial.
Cell Culture Applications
Useful for cell biologists needing a sterile environment and a reliable medium for maintaining cell integrity.

Environmental Testing
Unsuitable for field applications where natural conditions and variations in salinity and pH are essential.

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PBST [10X]; Phosphate Buffered Saline with Tween-20 Solution; 80mM Na2HPO4, 1.5M NaCl, 20mM KH2PO4, 30mM KCl, 0.5% Tween-20, pH 7.4-1 L

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Pregunta: ¿Cómo comprar PBST [10X]; Phosphate Buffered Saline with Tween-20 en línea desde Ubuy?

Respuesta: Es fácil comprar PBST [10X]; Phosphate Buffered Saline with Tween-20 en línea desde Ubuy.. Solo tiene que buscar el producto, elegir su método de envío al pagar y recibirlo en su ubicación.
Pregunta: ¿Está PBST [10X]; Phosphate Buffered Saline with Tween-20 disponible para comprar en línea en Paraguay?

Respuesta: Sí, en Ubuy Paraguay, este producto está disponible para que lo compre a un precio razonable.. El PBST [10X]; Phosphate Buffered Saline with Tween-20 no está disponible localmente, pero puede confiar en nosotros con nuestros servicios de envío exprés.
Pregunta: ¿Cuánto tiempo se tarda en obtener el producto después de realizar el pedido?

Respuesta: El tiempo de entrega de su producto pedido varía según lo que haya pedido y el método de envío que haya elegido.. El tiempo de entrega estimado se menciona durante el proceso de pago, así que no se preocupe mientras compra.

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Limitaciones: Para los productos enviados al extranjero, ten en cuenta que cualquier garantía del fabricante puede no ser válida; las opciones de servicio del fabricante pueden no estar disponibles; los manuales del producto, las instrucciones y las advertencias de seguridad pueden no estar en los idiomas del país de destino; los productos (y los materiales que los acompañan) pueden no estar diseñados de acuerdo con las normas, especificaciones y requisitos de etiquetado del país de destino; y los productos pueden no ajustarse al voltaje del país de destino y a otras normas eléctricas (lo que requiere el uso de un adaptador o convertidor, si procede). El destinatario es responsable de asegurarse de que el producto puede ser importado legalmente al país de destino. Cuando hagas un pedido a Ubuy o a sus filiales, el destinatario es el importador registrado y debe cumplir todas las leyes y normativas del país de destino.
No todos los productos que aparecen en Ubuy están a la venta, ya que Ubuy es un motor de búsqueda a nivel mundial. Los productos están sujetos a las normas de exportación/comercio.

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características y beneficios

Comprehensive range of biological buffers for research and diagnostics.
Manufactured in a certified facility adhering to strict quality controls.
Offers 10X Concentrated solution of Phosphate Buffered Saline with Tween 20.
Ideal for wash solutions in Western blot and ELISA assays.
Formulated to effectively remove excess material without disrupting binding.
Ensures optimal performance in protein research and molecular biology studies.

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PBST [10X]; Phosphate Buffered Saline with Tween-20 Solution; 80mM Na2HPO4, 1.5M NaCl, 20mM KH2PO4, 30mM KCl, 0.5% Tween-20, pH 7.4-1 L

PYG 813767

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Detalles de producto

Who Should Buy?

DESCRIPCIÓN DEL PRODUCTO

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eCon Lab Supply Buffers Editorial Review

Customer Reviews & Ratings

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PYG 813767

características y beneficios

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